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TSKgel Reversed-Phase

Tosoh Bioscience offers a number of silica-based and polymer-based columns for reversed-phase applications such as pharmaceutical drugs, forensic compounds, derivatised amino acids, carbohydrates, steroids, lipids and fatty acids. Depending on their molecular weight, peptides may be analysed with the 80-140A silica-based columns, while proteins require a larger pore size column (200-300Å).

  •  Silica- and polymer-based materials
  •  Super series ideal for LC-MS
  •  High recovery for biomolecules


TSKgel Reversed-Phase material specifications

TSKgel PhaseFunctional GroupBonding Particle Size (µm) Pore Size (Å) Carbon Load (%)  EndcappedExclusion Limit (Da) 
Protein C4-300C4Polymeric33003Yes-
ODS-100VC18Monomeric3, 510015Yes8,000
ODS-100ZC18Monomeric3, 510020Yes8,000
ODS-80TMC18Monomeric5, 108015Yes6,000
ODS-80TSC18Monomeric5, 108015Yes6,000
ODS-120TC18Polymeric5, 1012022Yes10,000
ODS-120AC18Polymeric5, 1012020No10,000
OligoDNA RPC18Monomeric525010No165,000
Octadecyl-4PWC18Monomeric7, 13500--200,000
Phenyl-5PW RPPhenylMonomeric10, 131000--1,000,000


TSKgel Protein C4-300

TSKgel Protein C4-300 consists of a polymerically bonded butyl (C4) phase on 300Å ultra-pure silica. The latest surface modification techniques and endcapping of residual silanol groups, reduce undesirable secondary interactions and peak tailing. It has been designed for the optimal recovery and resolution of biomolecules such as recombinant proteins, antibody fragments or PEGylated proteins.


TSKgel ODS-140HTP columns were developed for use in high throughput (HTP) applications including drug discovery, pharmacokinetics and peptide digest separations. They are packed with 2.3µm particles, providing high resolution and short analysis times at moderate pressure. The lower pressure drop enables these columns to be used with either UHPLC (up to 9,000psi) or conventional HPLC systems.

TSKgel ODS-100V and ODS-100Z

Based on ultra-pure silica with a high surface area, TSKgel ODS-100V and ODS-100Z offer alternative selectivity to each other. TSKgel ODS-100V provides strong retention for polar compounds due to its lower C18 ligand density (15% carbon content). The phase is prepared by an incomplete first reaction with a difunctional octadecylsilane reagent, followed by endcapping with a mixture of two difunctional dialkylsilane reagents. This enables the phase to provide complete wetting and retention stability in 100% aqueous eluents.

TSKgel ODS-100Z contains a high density (20% carbon content) C18 bonded phase for maximum retention and selectivity of small molecular weight compounds. It is prepared by bonding the surface with a difunctional octadecylsilane, followed by repeated endcapping with monofunctional trimethylsilane reagent.

TSKgel Super series

The silica particles used in the Super series columns are monodisperse spherical 2.3µm beads which are polymerically bonded and exhaustively endcapped, to produce low metal-content, low silanol phases. The 2.3µm particle provides superior resolution and speed of analysis as well as improved sensitivity. The columns do not suffer from excessive pressure drops.

Polymer-based phases

The polymer-based reversed-phase materials are chemically stable from pH 2 - 12, allowing operation at basic pH where silica-based phases have limited chemical stability. The non-porous Octadecyl-NPR column produces extremely fast kinetics and quantitative recovery of proteins at sub-microgram loads. TSKgel Octadecyl-4PW is suitable for the analysis of peptides and small proteins, whereas the TSKgel Octadecyl-2PW is used for small pharmaceutical compounds at basic pH. TSKgel Phenyl-5PW has a high loading capacity and is ideal for the separation of high molecular weight proteins.

  • Literature: Tosoh RP brochure

Further technical information:


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