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TSKgel Hydrophobic Interaction (HIC)


TSKgel HIC packings consist of polymethacrylate base material, and a choice of three ligands (butyl, ether and phenyl) with varied hydrophobicities from low to high, respectively. The polymethacrylate base resin for the Ether-5PW and Phenyl-5PW columns is a porous material with a 1000A pore size and 1,000,000 Da exclusion limit (based on G5000PW resin), offering high capacity and high sample loads. The TSKgel Butyl-NPR features a non-porous 2.5um support. Shorter run times and excellent mass recovery make the Butyl-NPR column an ideal choice for high throughput process monitoring or QC analysis.

  • Ether, phenyl and butyl functionalities
  • Wide pH range (2 - 12)
  • Stable in either acid or caustic cleaning regimens
  • Fully scalable from analytical to preparative dimensions


 TSKgel HIC phases

PhaseStructureParticle Size (um) Pore Size (A)
Ether-5PW10, 13, 201000
Phenyl-5PW10, 13, 201000

 Column selection for TSKgel HIC columns

SampleMW Range (Da)TSKgel Column
Medium to large proteins>10,000Phenyl-5PW, Ether-5PW, Butyl-NPR
DNA, RNA and PCR products>500,000Phenyl-5PW, Butyl-NPR
Oligonucleotides>10,000Phenyl-5PW, Butyl-NPR


TSKgel Ether-5PW

TSKgel Ether-5PW has intermediate hydrophobicity. It is an excellent choice for separating hydrophobic molecules such as membrane proteins or monoclonal antibodies including IgG and IgM. 


TSKgel Phenyl-5PW

The most hydrophobic among the three TSKgel HIC columns, TSKgel Phenyl-5PW is an excellent choice to screen for the selectivity, retention and recovery of most biomolecules.


TSKgel Butyl-NPR   

The least hydrophobic of the HIC column offerings, TSKgel Butyl-NPR is the best choice for high speed separations with excellent recovery even for more hydrophobic samples. The non-porous resin requires lower sample loading and reduces analysis time. The ultra efficient 2.5um bead allows for fast, high efficiency separations ideal for time critical QC analysis or sample limited applications.


Further technical information:

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