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TSKgel SW size exclusion columns are widely used for aqueous analysis and isolation of proteins, antibodies, enzymes, nucleic acids and other biological macromolecules. Columns show high resolution and sensitivity and have excellent solvent stability with up to 100% polar organic solvents. The smaller particle size of the UltraSW and SWXL materials result in reduced analysis times. UP-SW3000 columns are now available for UHPLC-SEC applications improving productivity and resolution. All SW-type columns are available in stainless steel hardware; SWXL columns are also available in PEEK hardware. TSKgel SuperSW mAb HR is the ideal scouting column for unknown molecular weight compounds..

  • Rigid spherical porous silica
  • Hydrophilic bonding (diol groups)
  • Stable from pH 2.5 - 7.5
  • Widely referenced
  • Four different pore sizes

TSKgel Silica SEC phases

TSKgel SW PhaseParticle Size (µm)Pore Size (Å) Exclusion Limit (Da) for globular proteins
UltraSW Aggregate330010,000 - 2,000,000
SuperSW200041255,000 - 150,000
G2000SWXL51255,000 - 150,000
G2000SW10, 131255,000 - 150,000
UP-SW3000225010,000 - 500,000
SuperSW mAb HR425010,000 - 500,000
SuperSW mAb HTP425010,000 - 500,000
SuperSW3000425010,000 - 500,000
G3000SWXL525010,000 - 500,000
G3000SW10, 1325010,000 - 500,000
G4000SWXL845020,000 - 7,000,000
G4000SW13, 1745020,000 - 7,000,000


With modern advances in technology, Tosoh Bioscience have launched a range of 2um UHPLC columns for use in SEC. The TSKgel UP-SW3000 columns are available in 150 and 300mm lengths for use on modern, low dead volume HPLC or UHPLC instrumentation. They provide short analysis times and allow analysts to achieve improved resolution compared with the equivalent larger particle size columns. Offering the same exclusion range as the TSKgel G3000SWXL columns, these columns are ideal for fast separations of monoclonal antibody analysis or can be used to improve your separation

TSKgel UltraSW

Launched in 2013, TSKgel UltraSW columns contain spherical 3µm particles (300A pore size). TSKgel UltraSW Aggregate has a slightly larger pore size than the TSKgel SuperSW mAb columns and provides a wider separation window for higher order monoclonal antibody aggregates.

TSKgel SuperSW

In 1997, Tosoh Bioscience launched the initial TSKgel SuperSW columns which contain spherical 4µm particles in 30cm x 4.6mm id dimensions and extended this portfolio in 2013 with further columns including the SuperSW mAb HTP suitable for UHPLC applications. This range offers high sensitivity even with limited sample mass. The TSKgel SuperSW mAb HR - ‘HR' indicating high resolution - delivers superior resolution over the whole molecular weight range, from fragments to aggregates, when analysing monoclonals. It has the same column dimensions and molecular weight range as the well-established TSKgel G3000SWXL.


Introduced in 1987, the TSKgel SWXL column line were the 2nd generation TSKgel phases developed with 5 or 8µm particle size packings. The smaller particle sizes of the TSKgel SWXL columns mean these can be implemented to improve sample resolution or to reduce analysis time (over SW columns). The G3000SWXL has proven to be the industry standard for QC analysis of monoclonal antibodies. Columns feature high pore volume per unit column volume, low sample adsorption and high column efficiency, all contributing to excellent sample resolution.


The TSKgel SW column line, introduced in 1977, were the first of a long line of high performance Gel Filtration (GFC) columns that have become synonymous with analysing protein molecular weights. It features 10 or 13µm particle sizes with improved sample capacity for protein isolation due to the large pore sizes.

  • Literature: Tosoh SEC brochure


Further technical information:


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