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Ion Chromatography (IC) / Organic Acid / Carbohydrate Columns

Allsep Anion

Allsep Anion is a methacrylate based phase with quaternary ammonium functional groups, optimised for use with both suppressed and non-suppressed conductivity detection. Columns are compatible with common IC mobile phases, such as carbonate, bicarbonate, p-hydroxybenzoic acid, phthalic acid, succinic acid and sodium octane sulfonate.

Allsep Anion is recommended for applications involving inorganic anions, weak and strong acid ions, metal complexes and organic acids. It meets the requirements for the EPA method 300.0 Part A for determining inorganic ions in water.


The general features of Allsep Anion include:

  • 7µm polymer based anion exchange phase
  • Suppressed or non-suppressed conductivity detection
  • pH range 2 - 10
  • Use with 0 - 100% organic modifier
  • USP L23

Hichrom Alltech Anion/S IC

Hichrom Alltech Anion/S columns are based on 10um silica with quaternary ammonium ion-exchange groups. These columns are optimised for non-suppressed conductivity detection and are best suited for routine separations of chloride, bromide, nitrate and sulphate.

  • Silica-based for symmetrical peak shapes
  • Separates inorganic and organic anions

Hichrom Alltech Organic Acid

Hichrom Alltech Organic Acid columns are ion exclusion columns packed with sulphonated polystyrene-divinylbenzene. The OA-1000 and OA-2000 columns exhibit excellent selectivity for aliphatic and aromatic acids. As with most ion exclusion columns, a column heater in necessary for normal operating procedures.
The IOA-1000 and IOA-2000 columns are suitable for the separation of citric and other acids from glucose and fructose.

  • Rapid analysis of organic acids and alcohols
  • pH stable polymer resin
  • Isocratic 100% aqueous mobile phases only, no organic solvents
  • USP L17

Phase Specifications

Organic Acid PhaseParticle Size (μm)Column DimensionsApplications
300 x 6.5mmInorganic ions, such as fluoride, arsenate, sulphite, alcohols and most organic acids
100 x 6.5mmOrganic acids with low pH values, low MW straight chain acids and aromatic acids
300 x 7.8mmAcids of the tricarboxylic acid cycle (Krebs cycle)
150 x 6.5mmFast separation of acids and some alcohols

Hichrom Alltech Anion Exclusion

Hichrom Alltech Anion Exclusion columns are based on a highly sulphonated polystyrene-divinylbenzene cation-exchange resin. The phase has a particle size of 10μm and is designed for the separation of organic acids and weakly ionised anions by an anion exclusion mechanism. Typical mobile phases contain dilute mineral acids. Acetonitrile (<10%) may be added as organic modifier to decrease the retention of hydrophobic compounds.
Separates organic acids and weak acid anions

  • Polymer-based for broad pH stability
  • USP L22
  • Stainless steel columns are available with dimensions of 300 x 7.8mm and 100 x 7.8mm.

Hichrom Alltech Carbohydrate Cation

The Hichrom Alltech Carbohydrate Cation column consists of a highly efficient sulphonated polystyrene resin supplied in the calcium form. This column provides excellent separations using only water as the mobile phase.

  • Sulphonated polystyrene resin in the calcium form
  • 100% water used as mobile phase
  • Column heating required
  • USP L19

Analytical columns are available in dimensions 300 x 6.5mm.

 Please contact Hichrom regarding advice relating to other Grace ion chromatography columns. 

For further technical information:

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