Tosoh Bioscience: Affinity Chromatography

Tosoh Bioscience is an acknowledged leader in the field of bioseparations. Established over 22 years ago as TosoHaas, their TSK-GEL & TOYOPEARL chromatographic resins are important components in the analysis, isolation and purification of biomolecules.

Affinity Chromatography

Toyopearl polymeric AFC resins are available in a variety of group specific ligands. In addition, activated and reactive forms are available for the immobilization of target specific ligands. In AFC the mechanism of binding and elution varies depending on the immobilized ligand used. In some cases such as with Toyopearl AF-Chelate-650M (for Immobilized Metal Affinity Chromatography or IMAC) the binding and elution mechanism may change through the use of different metals or chemical modifiers. DBC values for AFC materials vary with ligand and are typically on the low side. However, because AFC has high selectivity and most of the capacity is used for the target, with feedstock impurities remaining unbound, AFC resins are typically used for the Capture and Intermediate purification steps of a manufacturing process.

Toyopearl Resins for AFC

Activated Resins:

Toyopearl AF-Epoxy-650
Toyopearl AF-Tresyl-650

Reactive Resins:

Toyopearl AF-Carboxy-650
Toyopearl AF-Amino-650
Toyopearl AF-Formyl-650

Toyopearl Resins with group specific ligands - ready to use

Toyopearl AF-Blue HC-650
Toyopearl AF-Chelate-650
Toyopearl AF-Red-650
Toyopearl AF-Heparin HC-650

For further information - download the technical pdf:

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