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... from AkzoNobel

Kromasil® EternityTM- designed for longer column life, even under extreme pH conditions

Kromasil Eternity is based on a proprietary grafting technology, whereby the 100Å bare silica is modified by bonding with an organosilane layer. Under specific proprietary conditions, the organosilane layer penetrates the silica, which results in a merged organic/inorganic interfacial gradient. The pores are virtually returned to their original size resulting in a surface presenting both inorganic (-OH) and organic (-R) silicic acid moieties. It is this process step which gives Kromasil Eternity its extreme chemical stability, extending the pH range and column lifetime. This modified silica is then functionalised with monofunctional C18 or PhenylHexyl, followed by a proprietary endcapping process.
Kromasil Eternity Phases

Phase

Particle Size (μm)

Pore Size (Å)

Surface Area (m2/g)

Carbon Load %

Endcapping

pH Range

C18

2.5, 5

100

330

14

proprietary

1 - 12

PhenylHexyl

2.5, 5

100

330

12

proprietary

2 - 12



pH Variation in Method Development
The wide pH range offered by Kromasil Eternity enables greater selectivity flexibility when analysing ionisable compounds. Even under harsh pH conditions, Kromasil Eternity columns show long lifetimes. The test mixture used in Figure 7 contains a neutral, an acidic and a basic compound. Under alkaline conditions, the basic procaine is retained more than 3-nitrobenzoic acid. At pH 2.1, 3-nitrobenzoic acid is retained more than the other compounds. These changes in relative retention provide useful method development options.
Scale-up and Scale-down
Kromasil Eternity C18 and PhenylHexyl are available as 2.5 and 5μm particle sizes. These cover a wide range of applications, including classical analytical HPLC using 5μm in 250mm length columns and rapid HPLC using 2.5μm in 50mm length columns under both HPLC and UHPLC conditions. Kromasil Eternity is also a good choice for semi-preparative applications, due to its high efficiency and chemical stability. Figure 8 shows how easily a separation can be scaled down from 5μm to 2.5μm or scaled up to 21.2mm i.d. columns. Identical retention times are obtained despite the different particle sizes and column dimensions.








Contact Hichrom to request a Technical brochure:

Column dimensions available - Kromasil Eternity C18

Analytical Columns
2.5μm Phase Column Dimensions (mm)

50 x 2.1100 x 2.150 x 4.6100 x 4.6
C18EH2CLD05EH2CLD10EH2CLA05EH2CLA10
PhenylHexylEH2PXD05EH2PXD10EH2PXA05EH2PXA10

5μm Phase

 

Column Dimensions (mm)

 

50 x 2.1150 x 2.1
50 x 4.6100 x 4.6150 x 4.6250 x 4.6
C18E05CLD05E05CLD15E05CLA05E05CLA10E05CLA15E05CLA25
PhenylHexylE05PXD05E05PXD15E05PXA05E05PXA10E05PXA15E05PXA25

Semi Preparative and Preparative Columns
5μm Phase

Column Dimensions (mm)


50 X 10.0150 X 10.0250 X 10.050 X 21.2150 X 21.2250 X 21.2
C18E05CLP05E05CLP15E05CLP25E05CLQ05E05CLQ15E05CLQ25
PhenylHexylE05PXP05E05PXP15E05PXP25E05PXQ05E05PXQ15E05PXQ25

Other column dimensions available on request.