Tel: +44 (0) 118 930 3660

Short Courses  - 2017

Basic Concepts of HPLC ( 1 hour 45mins)

Suitable for everyone

Locations

Month

Cost

This short seminar is intended as a first introduction to ‘HPLC' and is suitable for anyone who wants to gain a basic understanding of this technique. The basis of the separation process and retention mechanisms will be covered, as well as how the important parameters of resolution, peak shape and efficiency are measured. An outline of HPLC hardware and different modes of separation will be considered. This course will provide a good foundation from which to progress to more advanced training.

  • London
  • Loughborough
  • Bio City Scotland



  • February
  • July
  • November



£55 


HPLC/UHPLC Column Care & Maintenance (1 hour 45mins)

For all analysts working in HPLC/UHPLC




This short seminar is suitable for all analysts working in HPLC/UHPLC and focuses on the preventable problems that commonly occur with LC columns. The course will help analysts to think about how they use columns, why they use them in that way and what the resultant effects might be on column lifetime, column performance and method reproducibility. The presentation includes details on identifying common HPLC/UHPLC column problems and considers possible solutions. Valuable preventative column care, including mobile phase, pH, temperature, column storage, filters & guards will be discussed. This course is ideal for those chromatographers involved in practical HPLC/UHPLC who want to ensure longer column lifetime and greater reproducibility in their methods.

  • London
  • Loughborough
  • Bio City Scotland
  • February
  • July
  • November

£55

HPLC Instrument Basics & Troubleshooting (1 hour 45mins)

For all analysts working in HPLC

This short course is suitable for all analysts working in HPLC. Predominately focusing on the HPLC instrument, it covers the essential concepts and operational principles of High Performance Liquid Chromatography. The course will look at the various modules of an HPLC instrument and will consider briefly how these work and what can go wrong with them. By understanding the problems that commonly occur, analysts will be in a better position to identify solutions and resolve or prevent HPLC method problems.

  • London
  • Loughborough
  • February
  • July

£55

UHPLC/HPLC Method Development Strategies to Exploit Selectivity: Influence of Stationary Phase (1 hour 45mins)

Some experience useful

In this seminar we review the importance of chromatographic selectivity from a theoretical and practical perspective and how this relates to analyte resolution for method development. With an understanding of selectivity and using a variety of chromatographic data, we discuss phase design principles and how it is possible to introduce functionality that enhances selectivity whilst maximising other desirable phase attributes such as phase stability, reproducibility and mechanisms of interaction. The resulting novel phases are explored for their improved chromatographic performance, selectivity and value in method development for a range of analytes and separations. Finally, method development approaches are discussed & pragmatic, simple method development platforms are illustrated based upon rational phase & eluent choice.

  • Reading
  • Birmingham
  • Manchester
  • March
  • June
  • October

£55

Exploring the Power of Eluent pH as a Selectivity Tool in HPLC/UHPLC Method Development (1 hour 45mins)

Some previous experience useful


Eluent phase pH can be a powerful tool for the chromatographer. Choosing buffer systems and analysis conditions that span an extended pH range (pH 2 - 11) can have an effect on the retention behaviour and provide alternative selectivity for many acidic and basic analytes. Changes in analyte retention from eluent pH can be helpful for screening systems and method development platforms where observing the number of components in samples is important before method optimisation is attempted. The chromatographic selectivity 'space' for various mobile phase and column permutations will be described for a range of acidic, basic and neutral analyte mixtures and practical hints and tips for ensuring repeatable and reproducible chromatography, when working with an extended pH range, will be included.

  • Reading
  • Manchester
  • March
  • October

£55

A Step-by-Step Approach for Method Development to Generate a Successful HILIC Separation  (1 hour 45mins)

Some Chromatography experience useful


Hydrophilic interaction liquid chromatography (HILIC) separations are gaining increased prominence in many application areas. The ability to retain neutral and ionisable polar species makes HILIC particularly popular for metabolomics and drug development studies. This seminar is designed to help the new HILIC chromatographer avoid the typical pitfalls that HILIC presents by establishing the critical parameters involved in HILIC separations. It will also demonstrate the relevance of a generally applicable selectivity-based method development platform designed for implementation across HILIC platforms. A worked method development example is included. Key topics include : Hints & tips on key aspects of HILIC method development, a step by step approach for method development in HILIC and an example HILIC method development using this protocol

  • Reading
  • Birmingham
  • Cambridge
  • Manchester
  • March
  • June
  • October

£55

Theory & Practice of using Solid-Core Technology (1 hour 45mins)

No previous experience necessary

Solid-core technology is the latest innovative particle technology for UHPLC/HPLC separations. This seminar examines the theory behind the increased efficiency, faster
analysis and lower back pressure claims, before discussing practical aspects for rapid method development with optimised work flows. There will be a focus on chromatographic selectivity with solid-core particles and how it can be exploited to
maximise analyte resolution for various analyte mixtures and classes.

  • Reading
  • March

£55

Introduction to Sample Preparation Techniques (1 hour 45mins)

No previous experience necessary

This short course is aimed at analysts who are required to develop assay methods for analytes in challenging matrices and would like a better idea of the very wide range of sample preparation techniques that are available. Starting with a few important pointers on sampling, the course will cover techniques that can be used to separate and pre-concentrate analytes based on polarity, solubility, charge, functionality, molecular size or a combination of two or more mechanisms. This course will help you to select an appropriate sample preparation strategy from the wide range of techniques that are available.

  • London
  • April

£55

Theory & Practice of Solid Phase Extraction (SPE) (1 hour 30mins)

For all analysts working with SPE

This short course will explain how to develop robust, efficient solid phase extraction methods. The main modes of separation in SPE will be described, as well as the important factors to consider during method development in relation to the analyte and the matrix. The best approach for selecting and optimising experimental conditions will be explained, allowing analysts to move quickly through method development to successful validation.

  • London
  • April

£55

Data Integrity Awareness (1/2 Day)

Some chromatography experience useful

This ½ day course is aimed at analytical chemists who need to familiarise themselves with the essentials of data integrity and gain a better understanding of this vital topic. This course provides essential background information on the subject and builds on this to provide a better overall understanding of the key requirements and consequences. This course will help you discover what you need to do in this important area to protect both yourself and your company.

  • London
  • April

£95

Practical Chiral Chromatography (1 hour 45mins)

No previous Chiral experience needed.

Some Chromatography experience useful

This short seminar is intended as an introduction to those scientists who are involved in chiral chromatography and want to use the technique to assist in their analysis. Specifically aimed at end-users in the field, this seminar will give an understanding of the processes involved and the current approaches to chiral analysis conducted in industry. Written from an end-users perspective, this course is ideal for those chromatographers involved in practical chiral method development and those who want to develop more robust methodology that is fit for purpose.

  • Harrogate
  • April

£55

Practical SFC (1hour 45mins)

Some chromatography experience useful

This short seminar is intended as an introduction for those scientists who are involved in or are
thinking of moving into the field of Supercritical Fluid Chromatography and want to use the
technique to assist in their analysis. It will give an understanding of the processes involved and
the current approaches to both chiral and achiral SFC analysis conducted in industry. Written from
an end-users perspective, this course is ideal for those chromatographers involved in practical
SFC method development and those who want to develop more robust methodology that is fit for
purpose.


  • Harrogate
  • April

£55

Techniques for Biomolecule Characterisation (1 hour 45mins)

No previous experience needed

This short seminar discusses chromatographic techniques suitable for the analysis of large molecular weight biomolecules. With an increased interest in the area of biotherapeutics and more regulations focused on these pharmaceuticals, possible methods to characterise biomolecules are required. Modes such as Size Exclusion (SEC), Ion exchange, Hydrophobic Interaction (HIC) and Affinity Chromatography will be covered for intact biomolecules. Techniques that may be more familiar to those analysts involved in small molecule analysis, such as reversed-phase LC-MS methods for protein digests will also be discussed, alongside new tools focused primarily on large molecules, for example SEC for monoclonal antibody monomer, fragment and aggregate separations. Course attendees will leave with a better understanding of the variety of techniques available to enable a more complete characterisation of biomolecules.

  • Loughborough
  • Cambridge
  • May
  • June

£55

The Analysis of Polar Molecules (1 hour 45mins)

Some chromatography experience useful

This course is suitable for analysts looking to explore different chromatographic techniques/modes applicable for the analysis of polar molecules. It will introduce you to a number of possible techniques that can be employed to retain polar molecules, generally hard to retain using C18 columns in reversed-phase solvent systems. It includes details of new column chemistries suitable for reversed-phase retention of polars, such as polar embedded columns, as well as alternative techniques including: ion pairing, mixed mode, Hydrophilic Interaction Liquid Chromatography (HILIC), Aqueous Normal Phase (ANP) and Supercritical Fluid Chromatography (SFC). This course allows you to explore the various options available to expand the polarity range of molecules that can be retained and discusses how these complement standard reversed-phase methodology.

  • Loughborough
  • Reading
  • May
  • October

£55

Superficially Porous Phases - Development and Benefits for HPLC/UHPLC ( 1hour 45mins)

Some chromatography experience useful

Superficially porous or core shell particle phases are becoming increasingly popular in today's laboratory. This course will introduce you to the development of solid core particle technology and the anatomy of a modern core shell particle. It will demonstrate how reduction in band broadening with these phases enables chromatographic separations to be performed with increased resolution and higher sensitivity, compared to fully porous phases. The range of particle sizes currently available for both small molecule and larger biomolecule separations will be discussed, along with parameters to be considered when transferring methods from fully porous to core shell phases. Examples will be shown to demonstrate how ultra-fast separations can be achieved without excessively high operating pressures. This course will enable you to develop faster HPLC/UHPLC analyses, thereby increasing laboratory productivity.

  • Loughborough
  • Reading
  • May
  • October

£55

An Introduction to LC Method Translations (1 hour 45mins)

Some chromatography experience useful

This short course introduces the analyst to isocratic and gradient method translations, including adjusting column dimensions (eg. scaling a method up or down); moving between different instruments (eg. transferring a method between sites) and modifying particle platforms (eg. between fully porous and solid-core silica particles). The course will introduce the theory and illustrate each situation with a number of practical examples including data for successful translation and common reasons for failed method translations. A ‘hints and tips' section will also be included.

  • Birmingham
  • June

£55

An Introduction to Prep HPLC - Practical Hints and Tips (1 hour 45mins)

For all chromatographers looking to work with Preparative LC

This short seminar is intended as an introduction to those scientists who are involved in analytical chromatography and want to understand the principles of scaling up to semi-preparative and preparative dimensions. Aimed at end-users in the field, this course covers the practicalities and pitfalls of specific considerations such as adjusting column dimensions, flow rate requirements and particle size choices. Practical examples will be used to illustrate successful scale-up and common reasons for failed method translations discussed.

  • Loughborough
  • Bio City Scotland
  • July
  • November

£55


The Technique of Gas Chromatography - in 3 parts (1 hour 45mins each)

No previous experience needed

Given appropriate instrumentation, gas chromatography is a most valuable technique, capable of separating and quantifying a wide variety of sample mixtures. Established GC practitioners may be expected to develop and apply their skills in Troubleshooting and in Method Development (subject to regulatory control). Before investigating these two important elements, it is essential that the fundamentals of the GC process are considered, possibly as a revision exercise. This combination of Fundamentals, Troubleshooting and Method Development in three separate parts, with a small degree of overlap, ensures that GC users at all levels of development can benefit from some revision before proceeding to the more advanced aspects, according to their personal objectives.

  • Loughborough
  • October

£55

Further and Bespoke Short Seminars Courses We are happy to discuss your training requirements with you and tailor courses to suit your exact needs. To obtain the training that best suits you and your company, simply contact us to discuss your requirements.

On-Site Training & Further Courses We can bring our seminars to your laboratory, reducing travel and accommodation costs and the inconvenience of scheduling dates around departmental requirements. Please contact us to discuss your specific requirements.

Consultancy in all areas of LC, GC & Sample Preparation As well as theoretical and hands on courses, we offer consultancy and advice from industry experts covering all areas of chromatography including HPLC, UHPLC, LC-MS, GC, GC-MS, Ion Chromatography, Sample Preparation and other areas. Please contact us to discuss our services.

Free Technical Support and Advice We provide free technical support and advice in all areas of chromatography. Our technical desk is staffed by experienced and approachable chromatographers, who provide impartial and helpful advice on a wide range of topics including:

HPLC/UHPLC troubleshooting advice, Application searches and support, Method development guidance, LC & GC, Column selection recommendations, General LC, GC, SPE, CE, TLC advice and assistance

Please contact out technical support desk for free and friendly advice with any problem or question or to discuss our services.

All course fees are exclusive of VAT and include all refreshments and course notes. All course fees must be paid prior to commencement of the course dates. Please enquire for on-site costs.

For further information on our HPLC Workshops please contact us