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TSKgel Ion-Exchange

 

Tosoh Bioscience offers a range of silica and methacrylate based high efficiency columns for analysis and isolation of biomolecules by anion-and cation-exchange chromatography. The BioAssist materials were created to provide higher capacity for larger proteins and have biocompatible PEEK hardware

  •   Silica- and polymer-based anion- and cation-exchange materials
  •  Range of pore sizes
  •  Porous and non-porous materials

 

TSKgel Ion-Exchange phases

TSKgel PhaseTypeFunctional GrouppH RangeParticle Size (µm)Pore Size (Å) Exclusion Limit (Da) for PEG
Silica-bases (SW)
DEAE-2SW WAX DEAE 2 - 7.5 5 125 10,000
DEAE-3SW WAX DEAE 2 - 7.5 10 250 30,000
CM-2SW WCX Carboxymethyl 2 - 7.5 5 125 10,000
CM-3SW WCX Carboxymethyl 2 - 7.5 10 250 30,000
SP-2SW SCX Sulphopropyl 2 - 7.5 5 125 10,000
Polymer-based (PW)
SuperQ-5PW SAX Trimethylamino 2 - 12 10,13 1000 1,000,000
DEAE-5PW WAX DEAE 2 - 12 10,13, 20 1000 1,000,000
BioAssist Q SAX Polyamine 2 - 12 10, 13 ~4000 >5,000,000
SP-5PW SCX Sulphopropyl 2 - 12 10, 13, 20 1000 1,000,000
CM-5PW WCX Carboxymethyl 2 - 12 10, 13 1000 1,000,000
BioAssist S SCX Sulphopropyl 2 - 12 7, 13 ~1300 ~4,000,000
Non-porous (NPR)
DEAE-NPR WAX DEAE 2 - 12 2.5 ~0 500
SP-NPR SCX Sulphopropyl 2 - 12 2.5 ~0 500
DNA-NPR SAX Trimethylamino 2 - 12 2.5 ~0 500
Q-STAT SAX Trimethylamino 3 - 10 7, 10 ~0 500
DNA-STAT SAX Trimethylamino 3 - 10 5 ~0 500
CM-STAT WCX Carboxymethyl 3 - 10 7, 10 ~0 500
SP-STAT SCX Sulphopropyl 3- 10 7, 10 ~0 500

 

Silica-based phases (SW)

Silica-based ion-exchange materials have a smaller pore size compared to the polymer-based phases, and are most suitable for analysing smaller molecular weight samples such as nucleotides, pharmaceuticals, catecholamines and small peptides.

Polymer-based phases (PW)

The porous methacrylate based phases are derived from G5000PW spherical particles, derivatised with DEAE, SP or CM functionalities. The SuperQ-5PW is a higher capacity strong anion-exchanger, as a result of the polyamine functional groups. All PW phases show excellent stability from pH 2 - 12 and offer good mass and activity recovery for enzymes, proteins, DNA, nucleic acids and polypeptides. A method developed on an analytical column can be reliably scaled up to preparative and process scale dimensions, using the same backbone chemistry.

BioAssist columns

TSKgel BioAssist Q columns contain particles with very large pores that are functionalised with polyamine groups to form a network structure. The dynamic binding capacity of BioAssist Q columns is high over a wide molecular weight range. TSKgel BioAssist S columns are packed with polymethacrylate particles possessing smaller pores functionalised with sulphopropyl groups. Columns are supplied in biocompatible PEEK hardware for analysis by HPLC or FPLC. TSKgel BioAssist phases are particularly suitable for the analysis of monoclonal antibodies, plasmids and other large proteins.

Non-Porous Resin (NPR) materials

The NPR ion-exchange materials such as DEAE-NPR, DNA-NPR and SP-NPR are based on methacrylate polymer of 2.5µm particle size. High efficiency, coupled with low sample capacity, make these columns suitable for fast analysis and micro-scale preparative isolation of DNA digests, and haemoglobin variants.

TSKgel STAT series

TSKgel STAT columns, enable high performance separations with short analysis times. These phases consist of monodisperse, non porous polymethacrylate resin particles, the surface of which consists of an open access network of multi-layered anion- or cation-exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a high loading capacity and a low operating pressure, compared with traditional non porous resins. TSKgel STAT 10µm particle size columns are ideally suited for rapid candidate screening or process monitoring. The smaller 7µm particle size columns are designed for high resolution separations of nucleic acids, mAb variants, PEGylated protein or protein aggregates.

Further technical information:

                   

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