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TSKgel Hydrophilic Interaction (HILIC)

By using a strongly polar bonded phase such as TSKgel Amide-80 or TSKgel NH2-100, polar compounds can be retained by hydrophilic interaction retention mechanisms using a mobile phase mixture of acetonitrile and buffer. In contrast to the retention behaviour seen in reversed-phase, in HILIC solutes are retained longer by increasing the percentage of acetonitrile. The selectivity of TSKgel NH2-100 differs from TSKgel Amide-80 allowing method development in this mode.

  • Covalently bonded carbamoyl and amino phases
  • Compatible with LC-MS
  • TSKgel Amide-80 shows unique retention mechanism for saccharide analysis

TSKgel Amide-80

TSKgel Amide-80 columns are well documented in the literature for HILIC separations of polar compounds. The phase consists of spherical silica particles that are covalently bonded with non-ionic carbamoyl groups. Columns can be operated over a wide range of eluent conditions for use with a broad range of sample polarities. Target applications include the analysis of hydrophilic biomolecules, including saccharides, glycans, oligosaccharides, peptides, nucleic acids and small molecules for drug discovery, that are weakly retained by reversed-phase chromatography. TSKgel Amide-80 is generally preferred over amino phases due to its greater long term stability making it ideal for LC-MS applications.

TSKgel NH2-100

The TSKgel NH2-100 phase is based on silica treated with a special endcapping procedure followed by the stepwise introduction of amino groups. Due to the high ligand density and large surface area, 3um TSKgel NH2-100 columns show high retention for very polar compounds, including carbohydrates, peptides, vitamins and polar drugs. In addition, it exhibits increased long term stability when compared with conventional amino phases.

 

Further technical information:

For individual advice please contact our Technical Support team