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TSKgel Affinity

Affinity chromatography offers the highest level of specificity and selectivity in biomolecular separations and purifications. TSKgel Affinity columns are based on G5000PW (5PW) porous resin and are available in three group-specific stationary phases and one activated phase (Tresyl-5PW). The availability of process scale media, based on TOYOPEARL HW-65 resin (65um) using the same polymer chemistry, enables easy scale-up. The choice of a specific ligand is determined by the expected interaction between sample and column ligand.

  • Choice of 4 affinity ligands
  • Rigid polymeric base resin with wide pH range
  • Products for analytical, preparative through to process scale
  • New Protein A affinity column

TSKgel Affinity phases

TSKgel Affinity phase

 Ligand type

Particle size (um)

Pore size (A)

pH range

Adsorption capacity

Protein A-5PW

Protein A (code-modi¬fied hexamer of the C domain)

20

1000

2.5 - 12

-

Boronate-5PW

m-Aminophenyl boronate

10

1000

2 - 9

40umol/ml resin for sorbitol

Chelate-5PW

Iminodiacetic acid

10

1000

2 - 12

-

Tresyl-5PW

Tresyl

10

1000

2 - 12

>60mg/g dry resin for soybean trypsin inhibitor

Protein A-5PW

TSKgel Protein A-5PW expands the line of TSKgel columns for antibody analysis with a high performance affinity chromatography column specifically designed for fast and accurate determination of monoclonal antibody (mAb) concentration during cell line selection or upstream biopro¬cess optimization and control

 Boronate-5PW

Under alkaline conditions the boronate anionic structure of the TSKgel Boronate-5PW can bind with 1,2 cis-diol groups, such as those in carbohydrates, carbohydrate-containing compounds and catecholamines. This interaction is enhanced in the presence of Mg2+ ions and inhibited by amine-containing buffers.

Chelate-5PW

TSKgel Chelate-5PW utilises the ability of iminodiacetic acid to chelate ions such as Zn2+ and Cu2+. Peptides and proteins containing histidine residues can adsorb to these chelated ions at neutral pH and then be eluted with buffer. Applications include immunoglobulins, transferrins, lectins, milk proteins and membrane proteins.

Tresyl-5PW

The Tresyl-5PW (2,2,2-trifluoroethanesulphonyl) requires activation with a user-selected ligand containing amino, thiol, phenol or imidazole groups. This results in a ‘custom’ affinity ligand. Principal applications include the selective purification of antigens after coupling of the appropriate antibody.

 

Further technical information:

or contact our Technical Support team