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Acclaim Reversed-Phase & HILIC

 

Acclaim HPLC phases are based on high purity, porous silica particles (2.3, 3 and 5um), with advanced and innovative column bonding technologies. This provides complementary selectivity, high column efficiencies and symmetrical peaks.

  • Ultra-pure, porous spherical silica
  • Novel surface chemistries for different selectivities
  • LC-MS compatible
  • Wide range of phases

Acclaim Reversed-Phase and HILIC phase specifications

Acclaim PhaseChemistryParticle Size (um)Pore Size (A)Surface Area (m2/g)Carbon Load (%)EndcappedpH Range
120 C18 C18 2.2, 3, 5 120 300 18 Yes 2 - 8
300 C18 C18 3 300 100 7 Yes 2 - 8
C8 C8 2.2, 3, 5 120 300 11 Yes 2 - 8
C30 C30 3, 5 200 200 13 Yes 2 - 8
Phenyl-1 Alkyl aromatic 3 120 300 13 Yes 2 - 8
PolarAdvantage Embedded sulphonamide 2.2, 3, 5 120 300 16 Yes 2 - 8
PolarAdvantage II Embedded amide 2.2, 3, 5 120 300 16 Yes 1.5 - 10.5
HILIC-10 Proprietary 3 120 300 8 No 2 - 8

Acclaim RSLC

2.2um columns for Rapid Separation Liquid Chromatography are designed for high throughput, high efficiency separations and are compatible with UHPLC and standard HPLC systems.

Acclaim C30

is designed to provide high shape selectivity for separating hydrophobic structurally related isomers and unique selectivity complementary to other reversed-phase columns. It is compatible with highly aqueous eluents.

Acclaim PolarAdvantage (PA)

features a patented bonding chemistry that incorporates a polar sulphonamide group with an ether linkage near the silica surface. Acclaim PA columns are compatible with 100% aqueous eluents and offer unique selectivity and good peak shape for acidic, basic and neutral analytes

Acclaim PolarAdvantage ll (PA2)

columns feature a patented surface chemistry that incorporates an amide-embedded polar group and multi-point attachment between the ligands and silica surface. This unique chemistry provides enhanced hydrolytic stability from pH 1.5 to 10.5 with 100% aqueous eluents. Acclaim PA2 is specifically designed to withstand high pH conditions, making it a good choice for the separation of both basic and acidic analytes.

Acclaim HILIC-10

is designed for separating highly hydrophilic molecules by HILIC. The phase is based on high purity silica covalently modified with a proprietary hydrophilic layer. Acclaim HILIC-10 is compatible with up to 20% aqueous eluent, while maintaining affinity for polar analytes. Columns can be operated in the primary HILIC mode or, alternatively, in normal-phase mode.
[The chromatogram (Fig 16 from page 235 Hichrom Cat 9) shows class separation by normal-phase, where triglycerides elute using 1% IPA, diglycerides using 4% IPA and monoglycerides using 13% IPA.]

Literature:

  • Acclaim PolarAdvantage HPLC Columns

     

Further technical information:

                     

or contact our Technical Support team