Tel: +44 (0) 118 930 3660

Hypersil Gold

 

Hypersil GOLD columns are based on high purity silica and a novel proprietary derivatisation and endcapping procedure, which reduces unwanted secondary and tertiary interactions of an analyte with the silica. Twelve different chemistry phases are available, all with 1.9, 3 and 5um particle size options, offering chromatographers flexibility in column choice, whether they are using conventional or ultra-high pressure LC systems.

  • Excellent peak efficiency and symmetry
  • Increased sensitivity and improved resolution
  • Wide variety of chemistries
  • 1.9 to 12um particles

 

 Hypersil GOLD phase specifications

PhaseFunctional GroupParticle Size (um) Pore Size (A)Surface Area (m2/g)  Carbon Load (%)Endcapped  pH Range USP Code
GOLD Proprietary 1.9, 3, 5, 12 175 220 10 Yes 1 - 11 L1
GOLD C8 Octyl 1.9, 3, 5 175 220 8 Yes 2 - 9 L7
GOLD C4 Butyl 1.9, 3, 5 175 220 5 Yes 2 - 8 L26
GOLD aQ Octadecyl 1.9, 3, 5, 12 175 220 12 Polar 2 - 9 L1
GOLD PFP Perfluorophenyl 1.9, 3, 5 175 220 8 Yes 2 - 8 L43
GOLD Phenyl Phenyl 1.9, 3, 5 175 220 8 Yes 2 - 8 L11
GOLD CN Cyano 1.9, 3, 5 175 220 4 Yes 2 - 8 L10
GOLD Amino Amino 1.9, 3, 5 175 220 2 No 2 - 8 L8
GOLD AX Polymeric amine 1.9, 3, 5 175 220 6 Yes 2 - 8 -
GOLD SAX Quaternary amine 1.9, 3, 5 175 220 2.5 - 2 - 8 L14
GOLD Silica - 1.9, 3, 5 175 220 - n/a 2 - 8 L3
GOLD HILIC Polyethyleneimine 1.9, 3, 5 175 220 6 2 - 8 -

Hypersil GOLD Selectivities 

In addition to the original Hypersil GOLD phase, eleven other phases are available, offering a range of selectivity options to optimise separations and maximise productivity. Hypersil GOLD shows C18-like USP L1 retention and selectivity. The phase is claimed to be stable over the pH range 1 to 11.

Hypersil GOLD C4

Hypersil GOLD C4 columns provide similar selectivity to C18 and C8 columns but with less retention. The shorter chain length and lower hydrophobic character make C4 a particularly useful phase for separation of hydrophobic polypeptides and small proteins.

Hypersil GOLD aQ

Hypersil GOLD aQ is a polar endcapped C18 phase, which is compatible with highly aqueous eluents. It offers superior retention of polar compounds, due to the polar functional group providing additional interaction mechanisms with polar compounds.

 

Hypersil GOLD PFP

Hypersil GOLD PFP columns offer alternative selectivity in reversed-phase HPLC by offering extra retention and selectivity for positional isomers of halogenated compounds. They are also well suited for the selective analysis of non-halogenated compounds, particularly polar compounds containing hydroxyl, carboxyl, nitro or other polar groups.

Hypersil GOLD Amino

Hypersil GOLD Amino can be used as a weak anion-exchange material for the analysis of anions and organic acids. It is also useful for carbohydrate analysis when used in reversed-phase or HILIC mode.

Hypersil GOLD AX

Hypersil GOLD AX columns utilise a novel polymeric amine ligand bonded to silica. It is a weak anion-exchange material, suitable for the analysis of smaller proteins and peptides. They are particularly suited to the analysis of polar compounds in HILIC applications.

Hypersil GOLD SAX

Hypersil GOLD SAX is a highly stable silica-based quaternary amine strong anion-exchange phase. Columns are suited to the analysis of smaller organic molecules such as nucleotides and organic acids.

Literature:

  • Hypersil GOLD Phase Overview
  • Hypersil GOLD HPLC Column Technical Manual

Further Hypersil GOLD technical information

                   

Or contact our Technical Support team