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Hypercarb

Typical C18 silica

Hypercarb

 

Hypercarb is a unique Porous Graphitic Carbon (PGC) phase composed of flat sheets of hexagonally arranged carbon atoms, with a fully satisfied valence. It has exceptional ability to retain very polar compounds and to separate closely related compounds such as isomers and homologous series, by a combination of hydrophobic and electrostatic retention mechanisms. Hypercarb is ideal for use with LC-MS and can retain and separate very polar compounds using ‘MS friendly' mobile phases.

  • Excellent retention of very polar analytes
  • Separates structurally related substances
  • pH stable from 0 to 14
  • Ideal for high temperature applications

Hypercarb phase specifications

Particle Size (um)Pore Size (A)Pore Volume (cc/g)Surface Area (m2/g) Carbon Load (%) 
3, 5, 7 250 0.7 120 100

Increased retention of polar analytes

The majority of reversed-phase systems show decreased retention as the analyte polarity increases. However, with Hypercarb, retention increases as analyte polarity increases for some analytes.

Resolution of structurally related compounds

Hypercarb can differentiate between closely related analytes such as isomers and homologous series. This ability is based on the analyte's fit to the graphite surface, allowing for the chromatographic resolution of compounds that are very similar in structure.

Extended pH range

Due to Hypercarb's unique characteristics, it is stable across the entire pH range of 0 to 14, enabling applications to be run at pH levels that are incompatible with most silica-based columns.

Ideal for LC-MS

Hypercarb columns can retain and separate very polar compounds using ‘MS friendly' mobile phases such as 0.1% formic or acetic acid and low concentrations of volatile buffers. High concentrations of organic modifiers in the mobile phase can be used, to improve sensitivity of atmospheric pressure ionisation techniques. Hypercarb is stable with any mobile phase and shows no phase bleed, as the porous graphitic surface is not modified. Shorter columns with smaller diameters can be used without compromising peak capacity and often resulting in increased sensitivity.

Columns are available in capillary to preparative dimensions.

Literature:

  • Method Development Guide for Hypercarb columns
 

Further technical information on Hypercarb columns:

                

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