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BioBasic

 

Thermo Scientific's BioBasic columns are designed for separations of proteins, peptides and nucleic acids. Available in a range of reversed-phase, ion-exchange and size exclusion phases, BioBasic columns are suitable for a variety of protein and peptide application needs from sample clean-up to analysis, including proteomics and LC-MS applications.

BioBasic Reversed-Phase columns

BioBasic reversed-phase columns are available in C18, C8 and C4 chemistries. These silica based columns provide superior chromatography because the extra dense bonding chemistry produces a highly stable, reproducible surface. Columns show excellent reproducibility and are ideally suited for LC-MS separations.

  • 300A pore size for improved performance of biomolecules
  • High performance, good efficiency and column lifetimes
  • Ideal for LC-MS applications

BioBasic RP phase specifications

BioBasic PhaseParticle Size (um)Pore Size (A) Surface Area (m2/g) Carbon Load (%)  Endcapped
18 5 300 100 9 Yes
8 5 300 100 5 Yes
4 5 300 100 4 Yes

 

BioBasic Ion-Exchange columns

BioBasic AX and SCX silica based columns show excellent performance for proteins, peptides and other ionic species and polar molecules. These ion-exchange materials can be used across a wide range of pH and ionic strength conditions.

In addition to its anion-exchange applications, BioBasic AX can also be used under high organic conditions in the HILIC mode. BioBasic SCX is a versatile strong cation-exchange phase for proteins, peptides and small molecules, also useful for protein fractionations for proteomics analyses by capillary LC-MS, including 2D proteomics combined with reversed-phase.

  • 300A pore size for enhanced protein and peptide separations
  • BioBasic AX for anion-exchange and HILIC
  • BioBasic SCX for cation-exchange

BioBasic Ion-Exchange phase specifications

BioBasic PhaseLigandParticle Size (um)Pore Size (A)Surface Area (m2/g)Carbon Load (%)
AX Polyethyleneimine 5 300 100 3
SCX Sulphonic acid 5 300 100 3

 

BioBasic SEC columns

BioBasic SEC columns, based on silica with a proprietary polymeric coating, offer the mechanical stability of silica-based size exclusion columns with higher efficiencies than those of polymer-based columns. Four pore sizes are available, making them ideal for molecular weight determination of peptides and proteins and for sample clean-up prior to other analyses.

  • Silica-based media for water soluble compounds
  • Pore sizes from 60 to 1000A
  • Ideal for sample clean-up

BioBasic SEC phase specifications

BioBasic PhaseParticle Size (um)Pore Size (A)Exclusion Limit (kDa) for proteins
SEC 60 5 60 0.1 - 6
SEC 120 5 120 0.1 - 50
SEC 300 2 3 1 - 500
SEC 1000 2 3 20 - 4000

 

Further information on BioBasic columns:

                     

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