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ACE Phases

ACE HPLC and UHPLC columns are designed to meet even the most challenging of chromatographic applications, giving excellent performance with acidic, basic and neutral molecules. A wide range of particle sizes, pore sizes and bonded chemistries and column dimensions is available in dimensions from capillary to preparative. Ultra high purity, ultra-inert ACE columns also provide excellent reproducibility and column lifetime.

ACE Excel columns (LINK to ACE Excel page), with increased pressure rating, are available for UHPLC/HPLC applications up to 1,000 bar (15,000 psi) in 1.7, 2, 3 and 5um particle sizes. ACE Excel columns are fully scalable with ACE HPLC columns.

  •  Ultra high purity base deactivated silica
  •  Wide range of standard and unique selectivity phases
  •  1.7, 2, 3, 5, 10 and 15um particle sizes
  •  Exceptional reproducibility
  •  Capillary to preparative dimensions

PhaseFunctional groupParticle size (um)Pore sizeSurface area (m2/g)Carbon load (%)EndcappedpH rangeUSP listing
C18-HL Octadecyl 3, 5, 10, 15 90 400 20.0 Yes 1.5-10* L1
C18 Octadecyl 1.7, 2, 3, 5, 10 100 300 15.5 Yes 1.5-10* L1
C8 Octyl 2, 3, 5, 10 100 300 9.0 Yes 1.5-10* L7
C4 Butyl 2, 3, 5, 10 100 300 5.5 Yes 1.5-10* L26
CN Cyano 2, 3, 5, 10 100 300 5.5 Yes 2-8 L10
Ph Phenyl 2, 3, 5, 10 100 300 9.5 Yes 1.5-10* L11
AQ Proprietary 2, 3, 5, 10 100 300 14.0 Yes 1.5-10* L1
SIL Unbonded 2, 3, 5, 10 100 300 - - 1.5-8 L3
C18-AR Octadecyl with integral Phenyl 1.7, 2, 3, 5, 10 100 300 15.5 Yes 1.5-10* L1
C18-PFP Octadecyl with integral PFP 1.7, 2, 3, 5, 10 100 300 14.3 Yes 1.5-10* L1
C18-Amide** Octadecyl with integral amide polar group 1.7, 2, 3, 5, 10 100 300 16.4 Yes 2-8 L1/L60
CN-ES** Cyano with proprietary extended alkyl spacer 1.7, 2, 3, 5, 10 100 300 12.6 Yes 2-8 L10
SuperC18** Octadecyl encapsulated 1.7, 2, 3, 5, 10 90 400 14.8 Yes 1.5-11.5* L1

* For optimum column lifetime, a pH range of 2-8 is recommended.

**Analytical columns are supplied in ACE Excel hardware format only

ACE C18

ACE C18 is the optimum and most versatile starting point for most method development activities. It offers ultra-inert silica for superior peak shape and guaranteed batch-to-batch and column-to-column reproducibility. ACE C18 provides hydrophobic interactions and some shape selectivity for general reversed-phase applications and analytes, including those differing in hydrophobicity and various charged and uncharged acids and bases.

ACE C18-AR

ACE C18-AR combines the hydrophobic characteristics of a C18 phase with the enhanced aromatic selectivity of a phenyl phase, allowing the benefits of both interactions to be fully exploited. The phase also exhibits ultra low bleed to ensure LC-MS compatibility and demonstrates excellent temperature and pH stability. Additionally ACE C18-AR is suitable for use in highly aqueous (up to 100%) mobile phases to enable the retention and separation of polar compounds. It is useful for analysing analytes with electron withdrawing and delocalised systems such as nitro groups, ketones, esters and acids.

ACE C18-PFP

ACE C18-PFP combines the hydrophobic characteristics of a C18 phase with the alternative selectivity offered by a pentafluorophenyl phase, providing pi-pi, dipole-dipole, hydrophobic and shape selectivity mechanisms. This phase is stable in 100% aqueous mobile phase conditions, exhibits ultra low bleed to ensure LC-MS compatibility and demonstrates excellent temperature and pH stability. ACE C18-PFP is ideal for halogenated analytes and analytes with electron donating groups such as phenols, aromatics, ethers and amines and is additionally useful for regioisomers.

ACE C18-Amide

ACE C18-Amideis a uniquely designed stationary phase offering improved retention of polar compounds, enhanced phenolic resolution and alternative selectivity to C18 bonded columns. The special design of the ACE C18-Amide ligand incorporates an extended alkyl chain spacer between the silica surface and amide polar group, which gives extra phase stability in eluents of pH 2 - 8. The longer C18 tail of the ligand increases the hydrophobic mechanism of interaction compared to phases with shorter (C14 - C16) tail groups. ACE C18-Amide offers low bleed for UV and LC-MS compatibility and can be used in 100% aqueous mobile phase conditions.

ACE CN-ES

ACE CN-ES combines CN polar selectivity with enhanced hydrophobicity, enabling the benefits of both interactions to be fully exploited. Extra phase stability and increased column lifetime is provided by an extended alkyl chain spacer between the silica surface and cyano group. ACE CN-ES is suitable for both reversed-phase and normal-phase separations and can be used in 100% aqueous mobile phase conditions.

ACE SuperC18

ACE SuperC18columns can be used in all instances where C18 columns would be used but offer improved inertness and excellent stability across an extended pH range (pH 1.5 to 11.5). ACE SuperC18 columns are designed for use with LC-MS compatible buffers and offer ultra-low bleed for improved LC-MS compatibility. They can be used with both methanol and acetonitrile mobile phases and offer rapid column equilibration without memory effects.
[Fig 2 from page 59 Hichrom Int. Catalogue].

Other ACE ultra-inert phases include C8, C4, C18-HL, CN, Phenyl, AQ and SIL.

Literature available

Further technical information: