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ACE Wide Pore Phases

ACE wide pore phases are based on 300A ultra inert silica. As for all ACE phases, their extremely low silanol activity results in excellent peak shape and reproducibility when separating compounds that contain polar functional groups, especially amines. This makes ACE 300A phases an ideal choice for the separation of peptides, proteins and other high molecular weight biomolecules. ACE 300A columns are available in an extensive range of dimensions and particle sizes for use in micro-scale separations, LC-MS analyses and high speed preparative analyses up to process scale1.7, 2, 3 and 5um particle size columns

  • 300A pore size ultra high purity silica
  • Ultimate protein and peptide application column
  • C18, C8, C4, CN and Phenyl chemistries
  • 3,5 and 10um particle sizes
  • Exceptional chemical stability



ACE Material Specifications

PhaseFunctional Group
Particle Size (um) Pore Size (A) Surface Area (m2/g) Carbon Load (%)
EndcappedUSP Listing
C18-300
Octadecyl
3, 5, 103001009.0YesL1
C8-300
Octyl
3, 5, 103001005.0YesL7
C4-300
Butyl
3, 5, 10300
1002.6YesL26
CN-300
Cyano
3, 5, 103001002.6YesL10
Ph-300
Phenyl
3, 5, 10300100
5.3YesL11

ACE 300A phases maintain good separation characteristics even when using low levels (e.g. 0.005%) of TFA, leading to increased sensitivity of mass spectral detection.
[These chromatograms (Fig 15 page 67 Hichrom Int cat) demonstrate this.]

The ability of TFA and other eluent additives to complex with peptides and proteins can be used to adjust selectivity and improve resolution.
[These chromatograms (Fig 16 page 67 Hichrom Int cat.) illustrate the selectivity of ACE C18-300 as a function of TFA concentration.]

In addition to the extensive range of analytical (1.0 to 4.6mm i.d.) through to preparative (21.2 - 50mm i.d.) columns, ACE 300A columns are available in capillary (500 and 300um i.d.) and nano (100 and 75um i.d.) dimensions. These capillary and nano columns offer high sensitivity due to their low dispersion characteristics and are particularly relevant in the areas of bioanalytical and proteomic analysis. They are ideal for use with detectors requiring very low flow rates, such as electrospray LC-MS

Literature available:

  • ACE catalogue
  • ACE C18-AR technical brochure
  • ACE C18-PFP technical brochure
  • ACE C18-Amide technical brochure
  • ACE CN-ES technical brochure
  • ACE SuperC18 technical brochure
  • A Guide to HPLC and LC-MS Buffer Selection
  • HPLC Troubleshooting Guide


Further technical information: