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ACE HILIC (HILIC-A, HILIC-B and HILIC-N)

HILIC provides the retention and separation of hydrophilic or polar to very polar analytes that are not well retained in RPLC. The ACE HILIC range comprises of three exciting selectivities for HILIC. These novel proprietary phases have been developed to enable chromatographers to achieve reproducible and successful HILIC separations using a systematic approach to method development.

  • 3 novel proprietary phases 
  • A simplified and systematic approach to HILIC method development
  • Compatible with all manufacturers' HPLC/UHPLC systems
  • Ultra robust up to 1,000 bar (15,000psi)
  • Three particle sizes (1.7, 3 and 5 micron)

ACE Material Specifications

 Phase

Functional group

Endcapped

Particle size (um)

Pore Size (A) 

Surface area (m2/g) 

Carbon load (%)

 pH range

USP listing

ACE HILIC-A

Propriatary SIL

No

1.7, 3, 5

100

300

-

2 - 7

L3

ACE HILIC-B

Proprietary aminopropyl

No

1.7, 3, 5

100

300

4.0

2 - 7

L8

ACE HILIC-N

Proprietary polyhydroxy

No

1.7, 3, 5

100

300

7.0

2 - 7

pending

The three phases comprise of an acidic character phase (ACE HILIC-A), a basic character phase (ACE HILIC-B) and a neutral character phase (ACE HILIC-N); each phase offering alternative selectivity. An ACE HILIC Method Development Techncial Guide is available that demonstrates a simplified and systematic approach to HILIC method development allowing chromatographers to achieve reliable, reproducibile HILIC methods. Cost effective Method Development kits, containing all three phases for the price of just a single column, are also available.

Literature available:

Further technical information: